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primary endothelial cells  (ATCC)


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    ATCC primary endothelial cells
    Primary Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aortic+endothelial+cells/Primary+Aortic+Endothelial+Cells%3B+Normal%2C+Human/10__1016_slash_j__isci__2026__116096-288-7-19
    Average 99 stars, based on 448 article reviews
    primary endothelial cells - by Bioz Stars, 2026-10
    99/100 stars

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    Reverse Transcription:

    Article Title: Fabricating microfluidic co-cultures of immortalised cell lines uncovers robust design principles for the simultaneous formation of patterned, vascularised, and stem cell-derived adipose tissue
    Article Snippet: .. Human telomerase reverse transcriptase (hTERT) immortalized adipose-derived mesenchymal stem cells (hAD-MSCs; ASC52telo, SCRC-4000, American Type Culture Collection ATCC) were routinely maintained as per manufacturer’s instructions in MSC Basal Medium (#500-040) supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum (#500-030), herein referred to as ‘complete MSC media’, and subcultured at a confluence of approximately 80%. hTERT immortalized green fluorescent protein-expressing human aortic endothelial cells (GFP-hAECs; TeloHAEC-GFP, CRL-4054, ATCC) were routinely maintained as per manufacturers instruction in Vascular Cell Basal Medium (#100-0303) supplemented with Endothelial Cell Growth Kit - VEGF (#100-041) and 33 μM phenol red (#999-001), herein referred to as ‘complete vascular cell media’, and subcultured at a confluence of approximately 80%. ..

    Article Title: Fabricating Microfluidic Co-Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell-Derived Adipose Tissue.
    Article Snippet: .. Cell Culture and Imaging: This project was supported by The University of Queensland’s Human Research Ethics Committee through approval 2021/HE002698: “Tissue culturemodels for in vitro optimization of engineered biomaterial scaffolds and bioprocess techniques”, as well as The University of Queensland’s Institutional Biosafety Committee through approval IBC/602E/ChemEng/2023: “Risk group 2 immortalized cell line activities.” Cell Culture and Imaging—Routine Cell Line Maintenance: Human telomerase reverse transcriptase (hTERT) immortalized adipose-derived mesenchymal stem cells (hAD-MSCs; ASC52telo, SCRC-4000, American Type Culture Collection ATCC) were routinely maintained as per manufacturer’s instructions in MSC Basal Medium (#500-040) supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs – Low Serum (#500-030), herein referred to as “complete MSC media”, and subcultured at a confluence of ≈80%. hTERT immortalized green fluorescent protein-expressing human aortic endothelial cells (GFP-hAECs; TeloHAEC-GFP, CRL-4054, ATCC) were routinely maintained as per manufacturers instruction in Vascular Cell Basal Medium (#100-0303) supplemented with Endothelial Cell Growth Kit – VEGF (#100-041) and 33 μM phenol red (#999-001), herein referred to as “complete vascular cell media”, and subcultured at a confluence of ≈80%. ..

    Article Title: Fabricating Microfluidic Co‐Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell‐Derived Adipose Tissue
    Article Snippet: .. Human telomerase reverse transcriptase (hTERT) immortalized adipose‐derived mesenchymal stem cells (hAD‐MSCs; ASC52telo, SCRC‐4000, American Type Culture Collection ATCC) were routinely maintained as per manufacturer's instructions in MSC Basal Medium (#500‐040) supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical‐derived MSCs – Low Serum (#500‐030), herein referred to as “complete MSC media”, and subcultured at a confluence of ≈80%. hTERT immortalized green fluorescent protein‐expressing human aortic endothelial cells (GFP‐hAECs; TeloHAEC‐GFP, CRL‐4054, ATCC) were routinely maintained as per manufacturers instruction in Vascular Cell Basal Medium (#100‐0303) supplemented with Endothelial Cell Growth Kit – VEGF (#100‐041) and 33 μM phenol red (#999‐001), herein referred to as “complete vascular cell media”, and subcultured at a confluence of ≈80%. ..

    Cell Culture:

    Article Title: Fabricating Microfluidic Co-Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell-Derived Adipose Tissue.
    Article Snippet: .. Cell Culture and Imaging: This project was supported by The University of Queensland’s Human Research Ethics Committee through approval 2021/HE002698: “Tissue culturemodels for in vitro optimization of engineered biomaterial scaffolds and bioprocess techniques”, as well as The University of Queensland’s Institutional Biosafety Committee through approval IBC/602E/ChemEng/2023: “Risk group 2 immortalized cell line activities.” Cell Culture and Imaging—Routine Cell Line Maintenance: Human telomerase reverse transcriptase (hTERT) immortalized adipose-derived mesenchymal stem cells (hAD-MSCs; ASC52telo, SCRC-4000, American Type Culture Collection ATCC) were routinely maintained as per manufacturer’s instructions in MSC Basal Medium (#500-040) supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs – Low Serum (#500-030), herein referred to as “complete MSC media”, and subcultured at a confluence of ≈80%. hTERT immortalized green fluorescent protein-expressing human aortic endothelial cells (GFP-hAECs; TeloHAEC-GFP, CRL-4054, ATCC) were routinely maintained as per manufacturers instruction in Vascular Cell Basal Medium (#100-0303) supplemented with Endothelial Cell Growth Kit – VEGF (#100-041) and 33 μM phenol red (#999-001), herein referred to as “complete vascular cell media”, and subcultured at a confluence of ≈80%. ..

    Article Title: Cold exposure increases aortic dissection risk through extracellular cold inducible RNA binding protein and toll like receptor 4 signaling
    Article Snippet: .. Human telomerase-immortalized human aortic endothelial cells (TeloHAEC, ATCC, CRL-4052) were cultured in Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041). ..

    Imaging:

    Article Title: Fabricating Microfluidic Co-Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell-Derived Adipose Tissue.
    Article Snippet: .. Cell Culture and Imaging: This project was supported by The University of Queensland’s Human Research Ethics Committee through approval 2021/HE002698: “Tissue culturemodels for in vitro optimization of engineered biomaterial scaffolds and bioprocess techniques”, as well as The University of Queensland’s Institutional Biosafety Committee through approval IBC/602E/ChemEng/2023: “Risk group 2 immortalized cell line activities.” Cell Culture and Imaging—Routine Cell Line Maintenance: Human telomerase reverse transcriptase (hTERT) immortalized adipose-derived mesenchymal stem cells (hAD-MSCs; ASC52telo, SCRC-4000, American Type Culture Collection ATCC) were routinely maintained as per manufacturer’s instructions in MSC Basal Medium (#500-040) supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs – Low Serum (#500-030), herein referred to as “complete MSC media”, and subcultured at a confluence of ≈80%. hTERT immortalized green fluorescent protein-expressing human aortic endothelial cells (GFP-hAECs; TeloHAEC-GFP, CRL-4054, ATCC) were routinely maintained as per manufacturers instruction in Vascular Cell Basal Medium (#100-0303) supplemented with Endothelial Cell Growth Kit – VEGF (#100-041) and 33 μM phenol red (#999-001), herein referred to as “complete vascular cell media”, and subcultured at a confluence of ≈80%. ..

    In Vitro:

    Article Title: Fabricating Microfluidic Co-Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell-Derived Adipose Tissue.
    Article Snippet: .. Cell Culture and Imaging: This project was supported by The University of Queensland’s Human Research Ethics Committee through approval 2021/HE002698: “Tissue culturemodels for in vitro optimization of engineered biomaterial scaffolds and bioprocess techniques”, as well as The University of Queensland’s Institutional Biosafety Committee through approval IBC/602E/ChemEng/2023: “Risk group 2 immortalized cell line activities.” Cell Culture and Imaging—Routine Cell Line Maintenance: Human telomerase reverse transcriptase (hTERT) immortalized adipose-derived mesenchymal stem cells (hAD-MSCs; ASC52telo, SCRC-4000, American Type Culture Collection ATCC) were routinely maintained as per manufacturer’s instructions in MSC Basal Medium (#500-040) supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs – Low Serum (#500-030), herein referred to as “complete MSC media”, and subcultured at a confluence of ≈80%. hTERT immortalized green fluorescent protein-expressing human aortic endothelial cells (GFP-hAECs; TeloHAEC-GFP, CRL-4054, ATCC) were routinely maintained as per manufacturers instruction in Vascular Cell Basal Medium (#100-0303) supplemented with Endothelial Cell Growth Kit – VEGF (#100-041) and 33 μM phenol red (#999-001), herein referred to as “complete vascular cell media”, and subcultured at a confluence of ≈80%. ..

    CRISPR:

    Article Title: MAPK Signaling and Angiopoietin-2 Contribute to Endothelial Permeability in Capillary Malformations
    Article Snippet: The droplet fluorescence was read in a QX200 droplet reader and analyzed in QuantaSoft software (BioRad). .. CRISPR-Cas9 gene editing was used to introduce the GNAQ p.R183Q mutation into htert-immortalized human aortic endothelial cells (teloHAECs, ATCC CRL-4052). .. Following IDT Alt-R Homology Directed Repair (HDR) protocol, TeloHAECs were nucleofected using Lonza 4D-Nucleofector (V4XP-5024, Lonza, Lexington, MA) with a solution containing preformed ribonucleoprotein (RNP) complexes combining IDT manufactured Alt-R sgRNA and Alt-R spCas9 enzyme (commercially available from Integrated DNA Technologies, Coralville, IA); homology directed repair (HDR) templates (IDT) for both GNAQ p.R183Q mutant and endogenous alleles and a GFPmax plasmid (available within Lonza V4XP- 5024 kit).

    Introduce:

    Article Title: MAPK Signaling and Angiopoietin-2 Contribute to Endothelial Permeability in Capillary Malformations
    Article Snippet: The droplet fluorescence was read in a QX200 droplet reader and analyzed in QuantaSoft software (BioRad). .. CRISPR-Cas9 gene editing was used to introduce the GNAQ p.R183Q mutation into htert-immortalized human aortic endothelial cells (teloHAECs, ATCC CRL-4052). .. Following IDT Alt-R Homology Directed Repair (HDR) protocol, TeloHAECs were nucleofected using Lonza 4D-Nucleofector (V4XP-5024, Lonza, Lexington, MA) with a solution containing preformed ribonucleoprotein (RNP) complexes combining IDT manufactured Alt-R sgRNA and Alt-R spCas9 enzyme (commercially available from Integrated DNA Technologies, Coralville, IA); homology directed repair (HDR) templates (IDT) for both GNAQ p.R183Q mutant and endogenous alleles and a GFPmax plasmid (available within Lonza V4XP- 5024 kit).

    Mutagenesis:

    Article Title: MAPK Signaling and Angiopoietin-2 Contribute to Endothelial Permeability in Capillary Malformations
    Article Snippet: The droplet fluorescence was read in a QX200 droplet reader and analyzed in QuantaSoft software (BioRad). .. CRISPR-Cas9 gene editing was used to introduce the GNAQ p.R183Q mutation into htert-immortalized human aortic endothelial cells (teloHAECs, ATCC CRL-4052). .. Following IDT Alt-R Homology Directed Repair (HDR) protocol, TeloHAECs were nucleofected using Lonza 4D-Nucleofector (V4XP-5024, Lonza, Lexington, MA) with a solution containing preformed ribonucleoprotein (RNP) complexes combining IDT manufactured Alt-R sgRNA and Alt-R spCas9 enzyme (commercially available from Integrated DNA Technologies, Coralville, IA); homology directed repair (HDR) templates (IDT) for both GNAQ p.R183Q mutant and endogenous alleles and a GFPmax plasmid (available within Lonza V4XP- 5024 kit).



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    Image Search Results


    The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

    doi: 10.3389/fcvm.2026.1740816

    Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

    Techniques: CCK-8 Assay, Migration, Control